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cdna ngs library preparation  (New England Biolabs)


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    New England Biolabs cdna ngs library preparation
    Cdna Ngs Library Preparation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 3787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+ngs+library+preparation/NEBNext+Ultra+DNA+Library+Prep+Kit+for+Illumina/pm40997504-77-24-28
    Average 98 stars, based on 3787 article reviews
    cdna ngs library preparation - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Quantitative Proteomics:

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9
    Article Snippet: .. Differential expression analysis in PBMCs and monocytes For mRNA profiling of PBMCs and monocyte subpopulations using RNA- seq, mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    RNA Sequencing:

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9
    Article Snippet: .. Differential expression analysis in PBMCs and monocytes For mRNA profiling of PBMCs and monocyte subpopulations using RNA- seq, mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9
    Article Snippet: .. For mRNA profiling of PBMCs and monocyte subpopulations using RNA-seq, mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Isolation:

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9
    Article Snippet: .. Differential expression analysis in PBMCs and monocytes For mRNA profiling of PBMCs and monocyte subpopulations using RNA- seq, mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: Monocyte subpopulation profiling indicates CDK6-derived cell differentiation and identifies subpopulation-specific miRNA expression sets in acute and stable coronary artery disease
    Article Snippet: .. For mRNA profiling using RNA-Seq mRNA of the same samples was enriched using the NEBNext® Poly(A) Magnetic Isolation Module (NEB) followed by cDNA NGS library preparation (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting library was controlled by use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) und quantified using KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9
    Article Snippet: .. For mRNA profiling of PBMCs and monocyte subpopulations using RNA-seq, mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: PSTPIP1 and pyrin, two key regulators of macrophage differentiation.
    Article Snippet: ER-Hoxb8 wildtype, Pyrin KO and PSTPIP1 KO macrophages were differentiated for 5 days and stimulated with 100 ng/ml LPS (Sigma-Aldrich) for 3 h and 2,5 μM ATP for 30 min or left untreated. .. RNA was isolated using the NucleoSpin Extract II Isolation Kit (Macherey-Nagel). mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100A8 and S100A9
    Article Snippet: .. For mRNA profiling of PBMCs and monocyte subpopulations using RNA-Seq, mRNA was enriched using the NEBNext® Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Next-Generation Sequencing:

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9
    Article Snippet: .. Differential expression analysis in PBMCs and monocytes For mRNA profiling of PBMCs and monocyte subpopulations using RNA- seq, mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: Monocyte subpopulation profiling indicates CDK6-derived cell differentiation and identifies subpopulation-specific miRNA expression sets in acute and stable coronary artery disease
    Article Snippet: .. For mRNA profiling using RNA-Seq mRNA of the same samples was enriched using the NEBNext® Poly(A) Magnetic Isolation Module (NEB) followed by cDNA NGS library preparation (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting library was controlled by use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) und quantified using KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100a8 and S100a9
    Article Snippet: .. For mRNA profiling of PBMCs and monocyte subpopulations using RNA-seq, mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: PSTPIP1 and pyrin, two key regulators of macrophage differentiation.
    Article Snippet: ER-Hoxb8 wildtype, Pyrin KO and PSTPIP1 KO macrophages were differentiated for 5 days and stimulated with 100 ng/ml LPS (Sigma-Aldrich) for 3 h and 2,5 μM ATP for 30 min or left untreated. .. RNA was isolated using the NucleoSpin Extract II Isolation Kit (Macherey-Nagel). mRNA was enriched using the NEBNext Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    Article Title: C/EBPδ-induced epigenetic changes control the dynamic gene transcription of S100A8 and S100A9
    Article Snippet: .. For mRNA profiling of PBMCs and monocyte subpopulations using RNA-Seq, mRNA was enriched using the NEBNext® Poly(A) Magnetic Isolation Module (NEB), followed by cDNA NGS library preparation (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using the KAPA Library Quantification Kit for Illumina (Roche).

    RNA sequencing:

    Article Title: Monocyte subpopulation profiling indicates CDK6-derived cell differentiation and identifies subpopulation-specific miRNA expression sets in acute and stable coronary artery disease
    Article Snippet: .. For mRNA profiling using RNA-Seq mRNA of the same samples was enriched using the NEBNext® Poly(A) Magnetic Isolation Module (NEB) followed by cDNA NGS library preparation (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). .. The size of the resulting library was controlled by use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) und quantified using KAPA Library Quantification Kit for Illumina (Roche).



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    Workflow of the study. (b) Quantification of heart weight (HW)/body weight (BW) ratio, n refers to number of animals. (c) Heatmap representation of myocardial transcripts differentially expressed by Gapmer-Bigheart. (d) Western blot analysis of endogenous Rcan1 isoforms and Gapdh as a loading control in hearts from mice receiving AAV9-luciferase or AAV9-BIGHEART, n refers to number of hearts. (e) Quantification of relative Rcan1 isoforms from (d). (f) Design of antisense DNA-tiling probes, grouped into ‘‘even’’ and ‘‘odd’’ sets based on their positions along mouse lncRNA Bigheart and workflow of the study. (g) <t>Complementary</t> <t>DNA-tiling</t> oligonucleotides efficiently and specifically retrieve lncRNA Bigheart from chromatin, n refers to number of independent pull-down experiments. (h) ChIRP-qPCR validation of peaks from Bigheart ChIRP signals in Rcan1 promoter regions, Gapdh served as negative control, n refers to number of independent pull-down experiments. (i) Tabular representation of proteins detected by mass spectrometry after ChIRP retrieval of lncRNA Bigheart from chromatin. (j) Bigheart ChIRP retrieves hnRNP- F and Hmgb1 proteins. As a negative control, Gapdh was not detected after Bigheart ChIRP. (k) Worfkflow of the experiment. (l) ChIP-seq profiles for H3K27me3, H3K4me3, <t>H3K27Ac,</t> <t>RNA</t> Pol2, Hmgb1 and ChIRP-seq profiles for lncRNA Bigheart carried out in cardiomyocytes on the murine Rcan1 gene. (m) Schematic representation of the model. * P < 0.05 vs corresponding control group; # P < 0.05 vs corresponding treatment (error bars are s.e.m.). Statistical analysis consisted of a One-way ANOVA followed by Tukey’s multiple comparison test (b, e) . Source data are provided as a Source Data file.
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    Workflow of the study. (b) Quantification of heart weight (HW)/body weight (BW) ratio, n refers to number of animals. (c) Heatmap representation of myocardial transcripts differentially expressed by Gapmer-Bigheart. (d) Western blot analysis of endogenous Rcan1 isoforms and Gapdh as a loading control in hearts from mice receiving AAV9-luciferase or AAV9-BIGHEART, n refers to number of hearts. (e) Quantification of relative Rcan1 isoforms from (d). (f) Design of antisense DNA-tiling probes, grouped into ‘‘even’’ and ‘‘odd’’ sets based on their positions along mouse lncRNA Bigheart and workflow of the study. (g) <t>Complementary</t> <t>DNA-tiling</t> oligonucleotides efficiently and specifically retrieve lncRNA Bigheart from chromatin, n refers to number of independent pull-down experiments. (h) ChIRP-qPCR validation of peaks from Bigheart ChIRP signals in Rcan1 promoter regions, Gapdh served as negative control, n refers to number of independent pull-down experiments. (i) Tabular representation of proteins detected by mass spectrometry after ChIRP retrieval of lncRNA Bigheart from chromatin. (j) Bigheart ChIRP retrieves hnRNP- F and Hmgb1 proteins. As a negative control, Gapdh was not detected after Bigheart ChIRP. (k) Worfkflow of the experiment. (l) ChIP-seq profiles for H3K27me3, H3K4me3, <t>H3K27Ac,</t> <t>RNA</t> Pol2, Hmgb1 and ChIRP-seq profiles for lncRNA Bigheart carried out in cardiomyocytes on the murine Rcan1 gene. (m) Schematic representation of the model. * P < 0.05 vs corresponding control group; # P < 0.05 vs corresponding treatment (error bars are s.e.m.). Statistical analysis consisted of a One-way ANOVA followed by Tukey’s multiple comparison test (b, e) . Source data are provided as a Source Data file.
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    Image Search Results


    Workflow of the study. (b) Quantification of heart weight (HW)/body weight (BW) ratio, n refers to number of animals. (c) Heatmap representation of myocardial transcripts differentially expressed by Gapmer-Bigheart. (d) Western blot analysis of endogenous Rcan1 isoforms and Gapdh as a loading control in hearts from mice receiving AAV9-luciferase or AAV9-BIGHEART, n refers to number of hearts. (e) Quantification of relative Rcan1 isoforms from (d). (f) Design of antisense DNA-tiling probes, grouped into ‘‘even’’ and ‘‘odd’’ sets based on their positions along mouse lncRNA Bigheart and workflow of the study. (g) Complementary DNA-tiling oligonucleotides efficiently and specifically retrieve lncRNA Bigheart from chromatin, n refers to number of independent pull-down experiments. (h) ChIRP-qPCR validation of peaks from Bigheart ChIRP signals in Rcan1 promoter regions, Gapdh served as negative control, n refers to number of independent pull-down experiments. (i) Tabular representation of proteins detected by mass spectrometry after ChIRP retrieval of lncRNA Bigheart from chromatin. (j) Bigheart ChIRP retrieves hnRNP- F and Hmgb1 proteins. As a negative control, Gapdh was not detected after Bigheart ChIRP. (k) Worfkflow of the experiment. (l) ChIP-seq profiles for H3K27me3, H3K4me3, H3K27Ac, RNA Pol2, Hmgb1 and ChIRP-seq profiles for lncRNA Bigheart carried out in cardiomyocytes on the murine Rcan1 gene. (m) Schematic representation of the model. * P < 0.05 vs corresponding control group; # P < 0.05 vs corresponding treatment (error bars are s.e.m.). Statistical analysis consisted of a One-way ANOVA followed by Tukey’s multiple comparison test (b, e) . Source data are provided as a Source Data file.

    Journal: bioRxiv

    Article Title: LncRNA Bigheart trans-activates gene expression in a feed forward mechanism that facilitates calcineurin-NFAT signaling in myocardial hypertrophy

    doi: 10.1101/2023.12.11.571094

    Figure Lengend Snippet: Workflow of the study. (b) Quantification of heart weight (HW)/body weight (BW) ratio, n refers to number of animals. (c) Heatmap representation of myocardial transcripts differentially expressed by Gapmer-Bigheart. (d) Western blot analysis of endogenous Rcan1 isoforms and Gapdh as a loading control in hearts from mice receiving AAV9-luciferase or AAV9-BIGHEART, n refers to number of hearts. (e) Quantification of relative Rcan1 isoforms from (d). (f) Design of antisense DNA-tiling probes, grouped into ‘‘even’’ and ‘‘odd’’ sets based on their positions along mouse lncRNA Bigheart and workflow of the study. (g) Complementary DNA-tiling oligonucleotides efficiently and specifically retrieve lncRNA Bigheart from chromatin, n refers to number of independent pull-down experiments. (h) ChIRP-qPCR validation of peaks from Bigheart ChIRP signals in Rcan1 promoter regions, Gapdh served as negative control, n refers to number of independent pull-down experiments. (i) Tabular representation of proteins detected by mass spectrometry after ChIRP retrieval of lncRNA Bigheart from chromatin. (j) Bigheart ChIRP retrieves hnRNP- F and Hmgb1 proteins. As a negative control, Gapdh was not detected after Bigheart ChIRP. (k) Worfkflow of the experiment. (l) ChIP-seq profiles for H3K27me3, H3K4me3, H3K27Ac, RNA Pol2, Hmgb1 and ChIRP-seq profiles for lncRNA Bigheart carried out in cardiomyocytes on the murine Rcan1 gene. (m) Schematic representation of the model. * P < 0.05 vs corresponding control group; # P < 0.05 vs corresponding treatment (error bars are s.e.m.). Statistical analysis consisted of a One-way ANOVA followed by Tukey’s multiple comparison test (b, e) . Source data are provided as a Source Data file.

    Article Snippet: Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB).

    Techniques: Western Blot, Luciferase, Negative Control, Mass Spectrometry, ChIP-sequencing, Comparison